A new product of synthetic hirudin based on Chlamydomonas reinhardtii

hirudin

Catalog number# 0007

Chlamydomonas reinhardtii is a green microalgae that is rich in nutrients, with a protein content of over 40% and a reasonable amino acid composition. It contains 8 essential amino acids and can be used as a dietary protein source. Unsaturated fatty acids have a high proportion, including omega-3 ω6、ω9 Fatty acids, cellulose content is much higher than that of spirulina and microalgae, while Chlamydomonas reinhardtii is also a high-quality source of chlorophyll and contains various trace elements required by the human body, such as calcium, magnesium, sodium, potassium, phosphorus, selenium, zinc, etc. Chlamydomonas reinhardtii has been classified as a "generally considered safe" substance by the US Food and Drug Administration and approved for use as a food ingredient in Singapore and Hong Kong. In 2022, the China's National Health Commission of China approved it as a new food raw material.

Research has found that hirudin can significantly reduce liver index, fat index, and MAD levels, increase SOD activity, and downregulate MCP-1 expression. In a hyperlipidemic mouse model, hirudin can significantly reduce the body weight of rats, regulate metabolic disorders, and effectively inhibit lipid accumulation in tissues. It was also found that hirudin could inhibit the formation of lipid plaque in mice, and its plaque area decreased significantly. Hirudin can reduce serum total cholesterol (TC), serum thyroglobulin (TG), and low-density lipoprotein cholesterol (LDL-C) levels, increase serum high-density lipoprotein (HDL-C) levels, and increase the activity of liver lipoprotein esterase (LPL) and liver esterase (HL), effectively regulating lipid metabolism disorders.

At present, recombinant hirudin on the market is very similar in amino acid sequence and structure to natural hirudin, but they are not sulfated at the 63rd tyrosine residue, which makes the inhibitory constant of recombinant hirudin on thrombin 90% lower than that of natural hirudin, and it is also prone to side effects such as bleeding.